Chromatrap benefit ratios of ChIP technology in the search for ground breaking -
Chromatrap reports 3 other client documents published in various journals evaluated by peers, how prestigious citing its solid state chromatin immunoprecipitation (ChIP) proprietary technology has enabled innovative research.
A recent study published in Drug Metabolism & Deposition look the role of structural changes chromatin in the regulation of ontogenesis of human CYP3A .
The article found that variable trajectories in the development of drug-metabolizing enzymes have contributed to differences between individuals susceptibility to chemical toxicity and adverse drug reactions, particularly in the early stages of life. The document examines the currently known factors associated with these inter-individual differences may be involved. Looking CYP3A4 and 3A7 discerned regulatory areas in individuals of different ages, histone modifications can be used for research on the structural dynamics of chromatin as elucidated using ChIP kits Chromatrap.
Search results published in the Journal of Immunology consider how alcohol moderately and HSF1 induces hsp70 stress protein and inhibits the pro-inflammatory cytokines resulting from tolerance to endotoxin . Researchers examine how binge or moderate exposure to alcohol leads to host defenses against infection impaired. This sensitivity appears to be due to compromised innate immune response. The study explores the molecular mechanism by which alcohol mediates immunosuppression this, but reached no firm conclusion. The Department of Medicine, University of Massachusetts Medical School show herein mechanistic role that cell stress proteins HSF1 and playing in hsp70 inhibition of alcohol mediation / MyD88 pathway TLR4.
A third document, Biochim Biophys Acta published, examines the results of research by the Centre for Molecular Medicine at the Xiangya Hospital in China who studied 'FOXM1 receiver and co-regulate androgen CDC6 gene transcription and DNA replication in cancer cells in the prostate ". cancer cells Studying prostate (CaP) The team examined the regulation of E2F and AR (androgen receptor) in the transcription CDC6, a key component of DNA replication initiation mechanism . FOXM1, which is a specific transcription factor of cellular proliferation, stimulated the transcription CDC6 in cooperation with RA, in addition to having an effect on E2F pathway. Both overexpression and knockdown approaches were used
Launched worldwide in 2012 -. Chromatrap ® ChIP technology of solid state was shown by an increasing number of research groups around the world to be more effective than conventional methods based heel. Indeed, the porous polymer in the solid phase functionalized with either protein A or G, provides a larger surface area for binding of the antibody to chromatin with very low nonspecific binding. In addition, it uses an approach of the spin column, providing high speed, the process and postponement advantages over sepharose beads or magnetic. DNA pull down with Chromatrap ® is up to 25 times longer than conventional methods, while the signal to noise ratio for DNA enrichment is 2 to 3 times better, even with low chromatin samples between 50 ng to 3000ng immunoprecipitation.
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